Plant
Exp
-- Brassica napus --
Plant Expression Portal
Portal
Search
Blast
Comparison
Specificity
Coexpression
Cross-species
Summary
Search gene expressions by sequence similarity
Program
blastn
blastp
blastx
Database
cDNA
protein
E-value
Word size
16
20
24
28
32
64
128
256
Gap costs
Existence:5; Extension:2
Existence:2; Extension:2
Existence:1; Extension:2
Existence:0; Extension:2
Existence:3; Extension:1
Existence:2; Extension:1
Existence:1; Extension:1
Match/mimatch score
1, -2
1, -3
1, -4
2, -3
4, -5
1, -1
Expression Level
Metric
TPM
?
Transcripts Per Million (TPM) is a normalization method for RNA-seq, should be read as "for every 1,000,000 RNA molecules in the RNA-seq sample, x came from this gene/transcript."
FPKM
?
Fragments Per Kilobase of transcript per Million mapped reads(FPKM) is a expression level normalization method which normalizes read count based on gene length and the total number of mapped reads.
Splicing profile
Metric
JCEC
?
The PSI value is calculated including both reads that span junctions defined by rmats (Junction Counts) and reads that do not cross an exon boundary (Exon Counts).
JC
?
The PSI value is calculated including only reads that span junctions defined by rmats (Junction Counts)
Read coverage
?
Only alternative splicing events with enough read coverage are included.
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Alignment